natural oils enhance il-10 and ifn-γ production by human pbmcs cultured with malassezia furfur
نویسندگان
چکیده
background: malassezia furfur is alipophilic yeast that causes skin disease. objective: to evaluate the level of il-10, ifn-γ and il-12p70 in co-incubation of m. furfur grown on different forms of natural oils with pbmcs of healthy individuals. methods: pbmcs were obtained from blood samples of normal volunteers. m. furfur was cultured in different culture media containing almond oil, fish oil, walnut oil, full-fat milk, and a fat-free medium; and the yeasts grown were harvested and used for co-incubation with pbmcs in vitro. the ifn-γ, il-10, and il-12p70 levels were measured at different time intervals using elisa methods. results: generally, ifn-γ and il-10 levels in the coincubation of yeasts with walnut oil group (wog) and fish oil group (fog) were higher than those in the almond oil group (aog) and full-fat milk group (ffmg). although the il-12p70 was higher in groups such as aog, fog, and wog; the increase was not statistically significant. conclusion: the results demonstrated that the type of fat used by m. furfur in the culture media can influence the immune response and increases ifn-γ and il-10 levels in an early time point of the culture system.
منابع مشابه
Natural Oils Enhance IL-10 and IFN-γ Production by Human PBMCs Cultured with Malassezia furfur
Background: Malassezia furfur is alipophilic yeast that causes skin disease. Objective: To evaluate the level of IL-10, IFN-γ and IL-12P70 in co-incubation of M. furfur grown on different forms of natural oils with PBMCs of healthy individuals. Methods: PBMCs were obtained from blood samples of normal volunteers. M. furfur was cultured in different culture media containing almond oil, fish oil,...
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عنوان ژورنال:
iranian journal of immunologyجلد ۹، شماره ۲، صفحات ۱۰۹-۱۱۸
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